|1,000 honeybees found dead in Tippecanoe County|
It is estimated that honeybees pollinate 80 percent of the country's insect crops, which counts for over $20 billion dollars worth of crops each year. Scientists around the globe are extremely concerned that they are dropping dead at an alarming rate while some from Purdue are sounding the alarm about it.
Purdue entomologist Greg Hunt recently told a WLFI news reporter his theory and Purdue Emeritus Professor Don Huber has graciously provided a copy of his theory and research to the LCJ after submitting it to the Center for Honeybee Research. We hope that you will take time to read about this growing crisis that affects every American. It should be of serious concern since it has reached our little corner of the world.
Now, the lives of bees in Tippecanoe County are being threatened.
“A thousand dead bees in front of a hive, right after somebody planted corn three-tenths of a mile away,” said Purdue entomologist Greg Hunt.
It’s called neonicotinoid. Hunt said farmers are planting corn seeds treated with this pesticide, but he said it’s the process used to lubricate the seeds for easier planting that makes them a danger to bees.
“The grower needs to put talc in the hopper to make the seeds flow well, but then some of the pesticide get’s mixed in with the talc,” said Hunt.
Hunt said the contaminated talc from the seeds drifts from the fields onto other plants. Making something as small as a dandelion, toxic for bees.
“It only takes a tiny bit. I calculated that 150 acres of corn seed has enough neonicotinoid to kill every bee hive in the county,” said Hunt.
The leads to the question — Are treated seeds a must for farmers?
You can access the rest of WLFI's story at this link:
There have been discussions about neonicotinoids, poor nutrition, Nosema, and mysterious viruses. Now a soil pathologist points a finger at a suspect that's completely under our nose.
The following was written to the Center for Honeybee Research regarding CCD. Dr. Huber’s profile is at the end of this article.
|Dr. Don Huber, Professor Emeritus, Purdue|
Is glyphosate a contributing cause of bee colony collapse disorder (CCD)?
(Submitted to the Center for Honeybee Research by Dr. Don M. Huber)
Bee colony collapse disorder (CCD) is a growing threat to the efficient production of fruits, vegetables and nut crops, in addition to the critical role of bees as pollinators for numerous seed crops (Neumann and Carreck, 2009; Wines, 2013). CCD is characterized as a loss of adult (worker) bees from the hive that leaves the queen and immature bees (brood) inadequately attended even though there is adequate honey and other food present (van Engelsdorp et al, 2006; Wikipedia, 2013). The etiology (reason) of CCD is listed as unknown (NFIC, 2013) although neonicotinamid insecticides have been implicated in several studies through disruption of the endocrine hormone system (van Engelsdorp et al, 2006; Tapparo et al, 2012; Wikipedia, 2013) that causes bees to become disoriented and fail to return to the hive (NPIC, 2013).
Acute poisoning and disease leaving dead bees in and around the hive can generally be ruled out, although there is sometimes an increased incidence of Nosema and European foul brood (EFB) in stressed colonies that could be contributing factors in some cases (Pettis et al, 2012). Mineral nutritional deficiency is also suspected as a contributing stress factor in CCD (Ahmed, 2012) and malnutrition is the only universal condition found in all cases of CCD even though there is honey and bee-bread generally in the hive. This could be because of toxicity to the Lactobacillus and Bifobacterium species in the honey crop that digest the nectar and render the honey and bee-bread digestible (Ahmed, 2012).
Perhaps a more problematic cause of CCD has been over looked even though it is the most indiscriminately and extensively used chemical in agriculture and the environment. This organic phosphonate chemical that has been overlooked is the estimated 880 million pounds of the popular, broad-spectrum, systemic herbicide glyphosate (also marketed as Roundup®) used for broadcast weed control in general right-of-ways, home gardens, crop production, fallow fields, understory weed control in groves, vineyards, orchards, and parks; and for aquatic weed control in ponds and lakes. It is almost universally used on millions of acres of Roundup Ready® alfalfa, canola, corn, cotton, soybeans and sugar beets. An additional, more recent use has been as a crop desiccant prior to harvest for barley, beans, peas, peanuts, sugar cane, wheat, and for late season weed control in other crops.
These uses have created an extensive exposure level throughout the year with especially high concentrations in plants, air, water and soil during primary bee foraging periods. The exposure, physiological damage, and biological impact of glyphosate are consistent with all of the known conditions related to CCD as shown in Table 1. Of all of the potential individual factors implicated in CCD, glyphosate is the only compound extensively used world-wide where CCD occurs that impacts all of them. That compound, again, is the patented mineral chelator (USPTO, 1964), herbicide, and antibiotic (USPTO, 2000), glyphosate. New studies refer to this compound as the most biologically disruptive chemical in our environment (Samsel and Seneff, 2013). (E. Note: Samsel and Seneff is worth reading the abstract on the link. You can download the entire PDF, which goes into the modern diseases glyphosate is creating.)
Table 1. Common characteristics of glyphosate with CCD.
Mineral chelator, lowers nutrients in plants Malnutrition (the only universal condition
for all CCD!
Antibiotic to beneficial bacteria Loss of Lactobacillus and Bifidobacteria
(esp. Lactobacillus and Bifidobacteria spp.) (critical beneficial bacteria for digestion)
(Low mineral content of plants)
Neurotoxin Neurological challenge
Endocrine hormone disruption Disoriented
Immune suppressant Suppressed immune system
Stimulates fungal pathogens Nosema increased
Present in honey, nectar and other plant products
Glyphosate is an organic phosphonate compound that was first patented as a broad-spectrum, cat-ionic metal chelator by Stauffer Chemical Company in 1964 (USPTO, 1964), as an herbicide by Monsanto Company in 1974 (USPTO, 1974), and as an antibiotic by Monsanto Company in 2000 (USPTO, 2000). All of these uses are based on its ability to ‘grab onto’ and form a chelate complex that immobilizes mineral nutrients such as Ca, Fe, Co, Cu, Mn, Mg, Ni, Zn, etc. (Glass, 1984). These metal nutrients serve as metal co-factors for various enzyme systems in plants, microorganisms, and animals. Once these metal nutrients are chelated by glyphosate in soil or plants, they become physiologically unavailable as co-factors for many enzymatic and other physiological functions.
The broad-spectrum toxicity of glyphosate to plants initially simplified weed control, especially with selectivity provided by genetically engineered glyphosate-tolerant (Roundup Ready®, RR) plants, so that glyphosate could be applied directly to the RR plants without killing them. This use has led to an estimated annual indiscriminate usage of 880 million pounds of this mineral-immobilizing herbicide and antibiotic in the US. There is nothing in the genetic engineering process, however, that does anything to the glyphosate that is applied to these plants that are foraged by bees.
Glyphosate is systemic in plants: As a phloem mobile chemical, glyphosate from foliar, stem, or root uptake is systemic in plants where it accumulates in flower and reproductive parts, root and shoot tips, and legume nodules (Huber, 2010; Johal and Huber, 2009). Much of the glyphosate will remain in the plant and it can accumulate over years in perennial plants such as alfalfa, vine, fruit, and nut crops and environmental perennial species. It is an active mineral chelator in the treated plant for as many as 8 to 15 days after application before becoming sequestered in flower parts, other meristematic tissues, or soil. As little as 12 gm/acre (1/40th of herbicidal rate and well below the general 12-16 % drift rate) inhibits root uptake and translocation of Fe, Mn, Zn and other nutrients so that plants exposed to glyphosate directly or through drift in air or water have lower nutrient content (Bellaloui et al, 2009, 2011; Bott et al, 2008, 2011; Cakmak et al, 2009; Eker et al, 2006; Huber, 2010, 2012; Zobiole et al 2012).
Minerals in glyphosate-tolerant plants may be impacted even more by glyphosate than those in non-tolerant plants since there is nothing in the genetic engineering that does anything to nullify the glyphosate and its chelating effect on mineral nutrients. Since plant products are the source of essential mineral nutrients, bees may become mineral deficient, malnourished, have a weakened immune system, and be more susceptible to infections and abiotic (environmental) stresses.
Direct toxicity of glyphosate: Glyphosate is not acutely toxic to bees, but is chronically toxic to animals, and, like the neonicotinamid insecticides, glyphosate is a neurotoxin and disrupts the endocrine hormone system at very low exposure rates (Antoniou et al, 2012; Gasnier et al, 2009) that are well below levels found in air, water, and, especially, plant tissues (Benbrook, 2012; Huber, 2012).
The 880 million pounds of glyphosate indiscriminately applied throughout the environment leaves glyphosate residues in plants and the environment that can lead to chronic diseases in animals such as autism, botulism, Parkinson’s, difficale diarrhea (Clostridium difficile), immune suppression, Salmonella and numerous other diseases (Krueger et al, 2012; Shehata et al, 2012).
Disruption of the endocrine hormone system is associated with birth defects. The wide-spread cultivation of glyphosate-tolerant crops (alfalfa, canola, corn, cotton, sugar beets) since 1996 and use as a preharvest desiccant since 2000 have greatly increased the use of glyphosate (Benbrook, 2012; Yamada et al, 2009) and subsequent contamination of air, water, soil, and plant products consistent with the incidence of CCD (NPIC, 2013; Wikipedia, 2013).
Antibiotic activity of glyphosate: Glyphosate is a strong antibiotic and toxic to microorganisms possessing the Shikimate physiological pathway (Johal and Huber, 2009; Kremer and Means, 2009; Krueger et al, 2012; Shehata et al, 2012; USPTO, 2000)). Many of these sensitive microbes include beneficial bacteria such as Lactobacillus spp. and Bifidobacterium spp. that suppress pathogens such as Clostridium, Salmonella, E. coli, Nosema, and American (Paenibacillus larvae) and European foul brood (Ahmed, 2012; Clair et al, 2012; Krueger et al, 2012; Shehata et al, 2013). In the absence of these beneficial protective bacteria, the pathogens increase along with the toxins they produce (Krueger et al, 2012; Shehata et al, 2012).
Various fungal pathogens are especially increased in activity and virulence by glyphosate (Johal and Huber, 2009); Kremer et al, 2009; Krueger et al, 2012). All Apis species possess a similar Lactobacillus and Bifidobacterium species microbiota within the honey crop that is critical for collecting and transporting nectar to the hive as well as for the production of honey and bee-bread (Ahmed, 2012). Glyphosate is highly toxic to both of these bacterial species that are necessary for digestion of food and protection from pathogens (Ahmed, 2012; Wikipedia, 2013).
Exposure opportunity: Glyphosate is indiscriminately applied throughout the bee foraging period and is in significant amounts in air, water, and many plant parts frequented by bees. Although not highly volatile, it becomes airborne as drift and on particulate matter with significant levels detected in rain and ground water (USGS, 2012). It is highly water soluble and a common contaminate found in surface water from drift, run-off, or direct application to water for aquatic weed control. It is systemic and persistent in plants with as much as 80% accumulating in meristematic plant tissues such as flowers and buds frequented by bees and is found in honey collected by bees from contaminated flowers. The extensive cultivation of the many glyphosate-tolerant plants has permitted the application of glyphosate before, during, after, and throughout the foraging period of bees to greatly expand the environmental and plant exposure of bees to this organic phosphonate chemical.
This proposal is initiated to determine if glyphosate is a contributing factor in CCD by analyzing exposure of bees to this chemical and its effect on the two predominate bacteria that are essential for bee nutrition and health (Ahmed, 2012). The focus on insecticides and their acute toxicity may have resulted in over-looking the direct and indirect chronic effects of glyphosate as a contributing factor to bee colony collapse disorder.
C: Some of us may not know what use “as a crop desiccant prior to harvest” means. Could you tell us why this is a new use - and what farmers are doing when they apply it like this?
L: Dr. Huber, is the spraying of glyphosate being used in addition to (used to dry up the soil) being used in no-till farming before planting to kill weeds- and Is there a concern to the possible combination of these two chemicals?
C: From what you’ve said, glyphosate builds up in the soil and in the tissues of plants which I presume are resistant enough not to die - so why are so many applications necessary? On a typical GMO crop farm, how many applications would there likely be in a year’s time?
L: Dr. would you elaborate on the statement regarding mineral nutritional deficiency and toxicity to the Lactobacillus and Bifobacterium- and how that might impact honeybees?
C: Dr. Huber, you mention that as little as 12 grams per acre of this chemical will affect the nutrient content of plants exposed. I wonder - if you could give us some comparative idea of what twelve grams looks like? And how much that is in comparison to a pound? You said there was more than 880 million pounds applied in the U.S. alone?
L: Can glyphosate accumulate in our bodies and over time also affect our immune systems, nervous systems, etc.?
L: Last year, one of my hives had a queen that marched across the combs for 3 months without laying one egg and acted drunk and nervous. Since I live in an area where a lot of GMO corn is being planted - could that have disrupted her egg-laying ability and made her nervous?
L: Dr. H, are you saying that in GMOs modified for glyphosate-tolerance, the blocked minerals are not getting in - as compared to plants that have not been genetically modified?
C: It’s scary to hear you say there are ‘significant levels detected in rain and ground water. Is glyphosate evaporating or being washed out of the sky? And could you tell us how this compares to the ‘safe’ exposure levels established by the EPA or whoever is in charge of safe-guarding the public?
Profile for Dr. Huber:
ü Dr. Huber is Professor Emeritus of Plant Pathology at Purdue University, West Lafayette, IN. He received B.S. and M.S. degrees from the University of Idaho (1957, 1959), a Ph-D from Michigan State University (1963), and is a graduate of the US Army Command & General Staff College and Industrial College of the Armed Forces. He was at the Department of Botany & Plant Pathology at Purdue University in 1971.
ü His agricultural research the past 50 years has focused on the epidemiology and control of soil-borne plant pathogens with emphasis on microbial ecology, cultural and biological controls, and physiology of host-parasite relationships.
ü He retired in 1995 as Associate Director of the Armed Forces Medical Intelligence Center (Colonel) after 41+ years of active and reserve military service.
ü Dr. Huber is an active scientific reviewer; international research cooperator with projects in Argentina, Australia, Brazil, Chile, China, Costa Rica, Denmark, Germany, Japan, Mexico, and Russia
ü He is internationally recognized for his expertise in the development of nitrification inhibitors to improve the efficiency of N fertilizers, interactions of the form of nitrogen, manganese and other nutrients in disease, herbicide-nutrient-disease interactions, techniques for rapid microbial identification, and cultural control of plant diseases.
ü Dr. Huber teaches courses on anti-crop bioterrorism and serves as a consultant on biological weapons of mass destruction and emerging diseases.
ü His greatest accomplishment has been his marriage to Paula Huber and their 11 children and 42 grandchildren and 2 great-grandchildren.
Proposed Research (Analyses to compare healthy with CCD)
1. Analyze for glyphosate (and AMPA) in pollen, honey (already shown), bee-bread, nectar and bees
2. Determine toxicity of glyphosate (rates) to Lactobacillus and Bifidobacterium species (already shown for other animals)
3. Endocrine hormone disrupter, neurotoxin, immune suppressant (already shown for other animals)
4. Glyphosate in CCD compared with normal (healthy) hives
5. Lactobacillus and Bifidobacterium in CCD compared with healthy hives, bees, brood (already shown absent in CCD)
Ahmed, N. 2012. Insect health: Lactic acid bacteria and honeybees. PLoS ONE 7(3):e33188. Doi: 10.1371/journal.pone.0033188
Antoniou, M., Robinson, C., and Fagan, J. 2012. GMO Myths and Truths: An evidence-based examination of the claims made for the safety and efficacy of genetically modified crops. Earth Open Source June 2012.
Bellaloui, N., Reddy, K.N., Zablotowitcz, R.M., Abbas, H.K., and Abel, C.A. 2009. Effects of glyphosate application on seed iron and root ferric (III) reductase in soybean cultivars. J. Agric. Food Chem. 57:9569-9574.
Bellaloui, N., Reddy, K.N., Bruns, H.A., Gillen, A.M., Mengistu, A., Zobiole, L.H., Fisher, D.K., Abbas, H.K., Zablotowicz, R.M., and Kremer, R.J. 2011. Soybean seed composition and quality: interactions of environment, genotype, and management. Pp. 1-42. IN: Maxwell, J.E. (ed.). Soybean: Cultivation, Uses, and Nutrition. Nova Science Publ.
Benbrook, C.M. 2012. Impacts of genetically engineered crops on pesticide use in the U.S. – the first 16 years. Environ. Sci. Europe 24:24 doi: 10.1186/2190-4715-24-24.
Bott, S., Tesfamariam, T., Candan, H., Cakmak, I., Roemheld, V., and Neumann, G. 2008. Glyphosate-induced impairment of plant growth and micronutrient status in glyphosate-resistant soybean (Glycine max L.) Plant Soil 312:1850194.
Bott, S., Tesfamariam, T., Kania. A., Eman, B., Aslan, N., Roemheld, V., and Neumann, G. 2011. Phytotoxicity of glyphosate soil residues re-mobilized by phosphate fertilization. Plant Soil 315:2-11. DOI: 10. 1007/s1104-010-0689-3.
Cakmak, I., Yazici, A., Tutus, Y., and Ozturk, L. 2009. Glyphosate reduced seed and leaf concentrations of calcium, magnesium, manganese, and iron in non-glyphosate-resistant soybean. European J. Agron. 31:114-119.
Clair, E., Linn, L., Trayert, C., Amiel, C., Seralini, G-E.,and Panoff, J.M. 2012. Effects of Roundup® and glyphosate on three food microorganisms: Geotrichum candidum, Lactococcus lactis subsp. cremoris and Lactobacillus delbrueckii subsp. bulgaricus. Cur. Microbiol. 64:486-491.
Eker, S., Ozturk, L., Yazici, A., Erenoglu, B., Roemheld, V., and Cakmak, I. 2006. Foliar-applied glyphosate substantially reduced uptake and transport of iron and manganese in sunflower (Helianthus annuus L.) plants. J. Agric. Food Chem. 54:10019-10025.
van Engelsdorp, D., Cox-Foster, D., Frazier, M., Ostiguy, N., and Hayes, J. 2006. Colony Collapse Disorder Preliminary Report (http://maaarec.cas.psu.edu/pressReleases/FallDwindleUpdate0107.pdf) Mid-Atlantic Apiculture Research and Extension Consortium (MAAREC) – CCD Working Group.
Gasnier, C., Dumont, C., Benachour, N., Clair, E., Chagnon, M-C., and Seralini, G-E. 2009. Glyphosate-based herbicides are toxic and endocrine disrupters in human cell lines. Toxicology 262:184-191.
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Huber, D.M. 2010. What’s new in Ag chemical and crop nutrient interactions. Fluid J. 18:3:14-16.
Huber, D.M. 2012. Plant nutrition and health risks associated with plant diseases. Chapt. 9, pp. 215-240. In: Bruulsema, T.W., Heffer, P., Welch, R.M., Cakmak, I., and Moran, K. (eds.). Fertilizing Crops to Improve Human Health: a Scientific Review. Vol. 3, Risk Reduction. International Plant Nutrition Institute, Norcross, GA.
Johal, G.H. and Huber, D.M. 2009. Glyphosate effects on diseases of plants. European J. Agron. 31:162-172.
Kremer, R.J. and Means, N.E. 2009. Glyphosate and glyphosate-resistant crop interactions with soil and rhizosphere microorganisms. European J. Agron. 31:153-161.
Krueger, M., Shehata, A., Neuhaus, J., Muller, T., Kotsch, M., and Schrodl, W. 2012. Chronic botulism of cattle, a multifactorial disease. What is the role of the herbicide glyphosate? Inst. Fur Bakteriol. Und Mykol., Univ. Leipzig, Germany.
National Pesticide Information Center. 2013. Bee Colony Collapse Disorder. http://npic.orst.edu/envir/ccd.html.
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Samsel, A. and Seneff, S. 2013. Glyphosate’s suppression of cytochrome P450 enzymes and amino acid biosynthesis by the gut microbiome: Pathways to modern diseases. Entropy 2012,14,1-x manuscripts; doi:10.3390/ el40x000x.
Shehata, A., Schrodl, W., Aldin, A.A., Hafez, H.M., Krueger, M. 2012. The effect of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro. Current Microbiol. DOI 10.1007/s00284-012-0277-2.
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Tapparo, A., Marton, D., Giorio, C., Zanella, A., Solda, L., Marzaro, M., Vivan, L., Girolami, V. 2012. Assessment of the environmental exposure of honeybees to particulate matter containing neonicotinoid insecticides coming from corn coated seeds. Environ. Sci. Technol. 46(5): 2592-2599. Doi: 10.1021/es2035152.Epub 2012 /Feb 17.
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United States Patent Office. Patent No. 7,771,736B2. Glyphosate formulations and their use for the inhibition of 5-enolpyruvyl-shikimate-3-phosphate synthase. [Glyphosase as an antibiotic for enteric organisms].
Wikipedia, 2013. Colony Collapse Disorder. http://en.wikipedia.org/wiki/Colony_collapse_disorder:1-29.
Wines, M. 2013. Mystery Malady Kills More Bees, Heightening Worry on Farms. New York Times March 28, 2013. http://www.nytimes.com/2013/03/29/science/earth/soaring-bee-deaths-in-2012-sound-alarm-on-malady.html?pagewanted=all)
Yamada, T., Kremer, R.J., Castro, P.R., and Wood, B.W. 2009. Introduction. Glyphosate interactions with physiology, nutrition, and diseases of plants: Threat to agricultural sustainability? European J. Agron. 31:111-113.
Zobiole, L.H., Kremer, R.J., Oliveira, R.S., Constantin, J. 2012. Effects of glyphosate application on photosynthesis, nutrient accumulation, and nodulation in glyphosate-resistant soybean. J. Soil Sci. Plant Nutr. 175:319-330.